Coding

Part:BBa_K187015:Design

Designed by: Team Biobytes   Group: iGEM09_Alberta   (2009-10-17)


Chloramphenicol resistance in pAB, Biobytes plasmid


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 10
    Illegal PstI site found at 655
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 655
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 10
    Illegal PstI site found at 655
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 10
    Illegal PstI site found at 655
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Note that the chlor resistance gene in this plasmid starts at the start codon and ends at the stop codon. A promoter and terminator are not included. pAB does include an RBS consensus sequence positioned 8 bp upstream of the ATG. We recommend that to express chlor resistance, you use the Biobytes assembly method together with the promoter and terminator parts we have submited in pAB and pBA to assemble promoters and terminators onto the chlor resistance gene. As there is a range of promoters to chose from, this allows rapid manipulation of gene expression level. Using the Biobytes method, several DNA segments can be combined in just 20min per segment.


Source

The choramphenicol resistance gene was cloned out of the plasmid pKD3. Biobytes plasmid pAB is entered as BBa_K187000.

References